Surface Area 10 Cm Dish

QUOTE (genehunter-1 @ Sep 15 2006, 07:35 PM)

A correction:

I remember the number listed for 100 mm dish is wrong. According to my measurement, the diameter for 100 mm dish is just 8.5 cm, not x cm the proper noun indicated. This translates to a surface expanse of ~ 5671 mm^ii, or ~75% of the surface of listed. And so multiply 0.75 to the numbers for 100 mm dish everyone!!!

I go suspicious when I saw the numbers of 100 mm dish and T75 flask. We all know a T75 is bigger than 100 mm dish, correct?

If the culture dishes are non 100mm, and then why are they notwithstanding called "100mm culture dish"?

-Minnie Mouse-

QUOTE (Minnie Mouse @ Sep 17 2006, 07:36 PM)

If the civilization dishes are not 100mm, then why are they nonetheless called "100mm culture dish"?

I recollect the size is based on the superlative embrace of the 10cm dish, wherease the base of the dish is the culturing area which is smaller than the encompass.

-scolix-

i retrieve it depends on the manufacturer...
yous can asking x, nine or even 11 cm dishes, and all are commonly named "x cm" but it is non ever exact!
bank check the product sheet
Seb

-tryptofan-

the pdf is very useful, thanks a lot

-T. reesei-

Depending on your cell type there could exist some not-density related bug. In a smaller well in that location is less space to motility effectually and the cells will observe the edges quicker which will have some furnishings on cell orientation...

-DLY-

QUOTE (pfmdr @ Aug 20 2006, 07:58 PM)

tin anybudy tell me

is there any effect of using different size of dishes for culturing

The size of dish does affair at times. Yous demand to use higher prison cell density for larger dishes. This is practiced if you desire more cells (for protein extraction, or other reasons). However, dish size does shows much departure if y'all are measuring the concentration of a biochemical in civilisation supernatant.

Regarding the pdf sent, its a very practiced ane. But, regarding 24-well plates, i have been using the cells at densities as high equally 1-5 million cells/well (i ml media per well) for abt four-6 days without whatsoever notable problem.

-rpsingh-

there is a stupid question I d like to ask: do the densities of the cell mean per cm2 or ml?

-subberry-

QUOTE (subberry @ Jun one 2007, 12:thirty AM)

at that place is a stupid question I d similar to ask: do the densities of the cell mean per cm2 or ml?

Not a stupid question at all, both are correct just they deal with the 2 dissimilar ways of growing cells. For example:-

J774/RAW 267.iv cells can grow in Suspension civilisation. Their density should not exceed 750,000 cells/ml.

If you lot grow the same cells on plastic i.due east. equally ADHERENT cells so the density of cells tin can be expressed as cells/cm2.

Hope this clears any defoliation. If you practise not inquire questions then you lot volition never acquire.

Regards

Rhombus

-Rhomb-

QUOTE (Rhombus @ Jun 1 2007, 04:xxx PM)

QUOTE (subberry @ Jun 1 2007, 12:30 AM)

there is a stupid question I d like to ask: do the densities of the prison cell hateful per cm2 or ml?

Not a stupid question at all, both are correct but they deal with the 2 different means of growing cells. For example:-

J774/RAW 267.four cells tin can grow in Suspension culture. Their density should non exceed 750,000 cells/ml.

If you abound the aforementioned cells on plastic i.e. as ADHERENT cells and then the density of cells can be expressed every bit cells/cm2.

Promise this clears any confusion. If you practise not ask questions then you volition never acquire.

Regards

Rhombus

Thank you for your warmhearted expalination. when I look in the tabular array attached in this topic(useful numbers for cell culture), I wander the jail cell number used in this table means past per cm2, right?

-subberry-

QUOTE (DLY @ May xvi 2007, 03:23 PM)

Depending on your cell blazon in that location could be some non-density related issues. In a smaller well there is less infinite to move around

yes.. i agree with that

QUOTE (DLY @ May 16 2007, 03:23 PM)

and the cells volition detect the edges quicker which will have some effects on cell orientation...

just, i dont really sympathise what this argument ways... is there anyone, perchance DLY, could explain ? but for curiosity sake... thank you a lot

-delimartin-